La suplementación de semen porcino descongelado con plasma seminal heterólogo no modifica la producción de radicales libres

  1. R. Fernandez Gago 1
  2. M. Mata Campuzano 1
  3. A. Besa 1
  4. M. Peñacoba 1
  5. C. Lorenzo 1
  6. J.C. Dominguez 1
  7. F. Martinez Pastor 1
  1. 1 Universidad de León
    info

    Universidad de León

    León, España

    ROR https://ror.org/02tzt0b78

Libro:
XV Jornadas sobre Producción Animal: 14 y 15 de mayo de 2013, Zaragoza
  1. Jorge Hugo Calvo Lacosta
  2. Isabel Casasús Pueyo
  3. Margalida Joy Torrens
  4. Javier Álvarez Rodríguez
  5. Luis Varona Aguado
  6. Begoña Panea Doblao
  7. Carlos Calvete Margolles
  8. Joaquim Balcells Teres

Editorial: Asociación Interprofesional para el Desarrollo Agrario

ISBN: 978-84-695-7684-7,  978-84-695-7684-7

Año de publicación: 2013

Volumen: 1

Páginas: 434-436

Congreso: Jornadas sobre producción animal (15. 2013. Zaragoza)

Tipo: Aportación congreso

DIALNET GOOGLE SCHOLAR lock_openAcceso abierto editor

Resumen

Supplementation with seminal plasma could improve the quality of cryopreserved boar semen. We have tested if a short incubation of post-thawed boar spermatozoa with 50% of homologous seminal plasma modify the levels of free radicals. We obtained five semen pools from frozen semen doses (three males) and evaluated the sperm viability (YOPRO1 or propidium iodide), the mitochondrial activity (Mitotracker deep red), the production of mitochondrial superoxide (MitoSOX) and the intracellular free radicals (CM-H2DCFDA) using flow cytometry. Samples were assessed just after thawing (control and adding 50% seminal plasma) and after 1 h of incubation at 37 °C. Incubation decreased viability, mitochondrial activity and mitochondrial superoxide production, with no significant effect of seminal plasma. The levels of intracellular free radicals did not change in the control, but they increased at 1 h in the 50% seminal plasma treatment. We could no detect a protective effect of seminal plasma in this study, but our results suggest an increase of intracellular free radicals with time. This could be associated to the stimulation of signalling pathways, which could be associated to the positive effects found in previous studies.

Fuente de los datos: Dialnet