Estudio "ex vivo" de la eficacia de la "autoinfusión" de plasma rico en células blancas en úlceras infectadas

Résumé

ntroduction: Infected diabetic foot ulcers (IDFUs) are recalcitrant ulcers with complicated clinical management. Platelet-rich plasma (PRP) infiltration is beneficial by promoting natural wound healing, but its composition is highly dependent on the concentration device and blood count of the subject. The role of leukocytes is decisive, they control infection and restore natural healing but it has not been examined. Objectives: To determine the effectiveness of the PRP rich in leukocytes (LRPRP) in DFIs and to optimize the therapy; antimicrobial activity, LRPRP variability between systems and subjects. Probability of clínical success. Material and Methods: Clinical and ex vivo trials were developed to determine the antimicrobial efficacy of LRPRP in prevalent organisms of DFIsand the compositional variation accordint to systems and subjects. Results: Achievable platelet and neutrophil concentrations in LRPRP reduce (> 2log CFU/ml, platelets) or contain (neutrophils) bioburden. The Easy Kit system is more optimal to obtain effective densities, although the cellular quality of LRPRP depends on the system and the sex and age of the subject. Conclusions: LRPRP would restore the natural healing process by reducing the bioburden of microorganisms. After optimizing therapy, any subject regardless of gender and age would benefit from treatment.