Efectos del medio de recuperación sobre la respuesta al calor de Bacilus stearothermaphilus

  1. Bernardo Álvarez, Ana
  2. González Prieto, Josefa
  3. González Martínez, Isaac
  4. López Fernández, Mercedes
  5. Martín Sarmiento, Roberto
Revista:
Anales de la Facultad de Veterinaria de León

ISSN: 0373-1170

Any de publicació: 1991

Any: 37

Número: 37

Pàgines: 29-40

Tipus: Article

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Altres publicacions en: Anales de la Facultad de Veterinaria de León

Resum

The effects of various recovery media: nutrient agar (NA), dextrose tryptone agar (DTA), antibiotic assay medium (AAM) and tripticase soy agar (TSA) on survival counts and DT and z values of heat treated spores of B. stearothermophilus were studied. NA, DTA and AAM provided very similar results in recoveries, DT and z, while, when TSA was used, a few higher z values and a 50% lower counts and DT values were obtained. The presence of sodium chloride in the plating media decrased the levels of recovery and DT values. Failure of different lots of nutrient agar to support the growth of B. stearothermophilus was due to a calcium deficiency in the medium.

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