Cinética de la reducción de la acetoina por la L-Glicol Deshidrogenasa (NAD+) de Enterobacter Aerogenes
- Vidal González, María Isabel
- Carballo García, Francisco Javier
- Bernardo Álvarez, Ana
- Martín Sarmiento, Roberto
- González Prieto, Josefa
ISSN: 0373-1170
Année de publication: 1989
Año: 35
Número: 35
Pages: 43-54
Type: Article
D'autres publications dans: Anales de la Facultad de Veterinaria de León
Résumé
Kinetics of the reversible reduction of acetoin to 2,3-butanediol by L-glycol dehydrogenate (NAD-+ from Enterobacter aerogenes was studied. Results obtained prove that the reaction proceeds via an Ordered Bi-Bi mechanism with the coenzyme as the "leading" substrate. This enzyme shows a high affinity by NADH and acetoin and a lower one by NAD and 2,3-butanediol.