Cinética de la reducción de la acetoina por la L-Glicol Deshidrogenasa (NAD+) de Enterobacter Aerogenes

  1. Vidal González, María Isabel
  2. Carballo García, Francisco Javier
  3. Bernardo Álvarez, Ana
  4. Martín Sarmiento, Roberto
  5. González Prieto, Josefa
Zeitschrift:
Anales de la Facultad de Veterinaria de León

ISSN: 0373-1170

Datum der Publikation: 1989

Jahr: 35

Nummer: 35

Seiten: 43-54

Art: Artikel

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Andere Publikationen in: Anales de la Facultad de Veterinaria de León

Zusammenfassung

Kinetics of the reversible reduction of acetoin to 2,3-butanediol by L-glycol dehydrogenate (NAD-+ from Enterobacter aerogenes was studied. Results obtained prove that the reaction proceeds via an Ordered Bi-Bi mechanism with the coenzyme as the "leading" substrate. This enzyme shows a high affinity by NADH and acetoin and a lower one by NAD and 2,3-butanediol.

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