Dos métodos rápidos para la purificación de la L-Glicol deshidrogenasa (NAD+) de Enterobacter Aerogenes

  1. Carballo García, Francisco Javier
  2. Martín Sarmiento, Roberto
  3. González Prieto, Josefa
  4. Bernardo Álvarez, Ana
  5. Rodríguez Salido, J. C.
Journal:
Anales de la Facultad de Veterinaria de León

ISSN: 0373-1170

Year of publication: 1989

Year: 35

Issue: 35

Pages: 31-41

Type: Article

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Abstract

Two new procedures to purify L-glycol dehydrogenase (NAD+) from Enterobacter aerogenes are proposed. Both of them give very pure preparations (s. a. = 31,5-34 I.U./ mg protein), with a high yield (42-49% of initial activity), avoid the use of fractionation techniques harmful to the enzyme and are also adventageous in requiring only 6-8 hours.

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