Dos métodos rápidos para la purificación de la L-Glicol deshidrogenasa (NAD+) de Enterobacter Aerogenes
- Carballo García, Francisco Javier
- Martín Sarmiento, Roberto
- González Prieto, Josefa
- Bernardo Álvarez, Ana
- Rodríguez Salido, J. C.
ISSN: 0373-1170
Datum der Publikation: 1989
Jahr: 35
Nummer: 35
Seiten: 31-41
Art: Artikel
Andere Publikationen in: Anales de la Facultad de Veterinaria de León
Zusammenfassung
Two new procedures to purify L-glycol dehydrogenase (NAD+) from Enterobacter aerogenes are proposed. Both of them give very pure preparations (s. a. = 31,5-34 I.U./ mg protein), with a high yield (42-49% of initial activity), avoid the use of fractionation techniques harmful to the enzyme and are also adventageous in requiring only 6-8 hours.