Papel de los canales TRPC6 y TRPA1 en la homeostasis del Ca²? intracelular

Abstract

Cells exert a variety of cell functions by regulation of cytosolic free Ca²? ([Ca²?]c). These functions include muscle contraction, platelet agregation, cell differentiation or apoptosis. Cellular agonists increase [Ca²?]c by releasing Ca²? from intracellular stores or by inducing Ca²? influx from extracellular medium. In non-excitable cells the main pathway of Ca²? entry is called "Store operated Ca²? entry (SOCE)", and it is activated by the filling state of the intracellular Ca²? stores. The three major proteins involved in SOCE are members of the families STIM, Orai and TRPC. This dissertation was focused on studying the role of TRPC6 and TRPA1 in SOCE. The results demonstrate that TRPA1 regulates negatively SOCE, by attenuation of STIM1-Orai1 association. Additionally, TRPC6 has also been reported to be sensitive to acidic conditions, and to participate in regulation of basal [Ca²?]c. Furthermore, STIM1, which is essential for SOCE, modulates TRPC6 heteromultimerization and its cellular location.