Estudio del perfil transcripcional de genes implicados en el metabolismo de los ácidos grasos de la leche en dos razas ovinas.

Resumen

Sheep milk ranks the fourth position in terms of global milk production from different species. Most of the world’s sheep milk is processed into dairy products, mostly cheese. Milk lipid synthesis as well as fatty acid esterification and milk fat secretion have a special interest due to their influence in manufacturing and organoleptic properties in dairy products. In this study we performed a RNA-seq analysis in two different dairy sheep breeds in order to evaluate the transcriptional profile of some genes implicated in the mammary gland fat metabolism along lactation. The selected genes are: LPL and VLDL, implicated in the fatty acid uptake from blood; ACACA, FASN and SCD, implicated in de novo synthesis and fatty acid desaturation; BTN1A1 and XDN implicated in lipid droplet formation. None of the genes evaluated is significantly differentially expressed between the two breeds nor along lactation, but we can see changes in the expression profile. Mostly all of the genes analyzed increased their expression along lactation. SCD1, ACACA y XDH were the most abundant key genes measured, appearing to be key genes in milk fat metabolism.