Estimación de la proteína no degradable en el rumen de pastos de la montaña leonesa empleando el CNCPS (Cornell)

  1. C. Valdés
  2. S. Andrés
  3. F.J. Giráldez
  4. R. García
  5. R. Peláez
  6. A. Calleja
Book:
Pastos, paisajes culturales entre tradición y nuevos paradigmas del siglo XXI
  1. López-Carrasco Férnandez, Celia (coord.)
  2. María del Pilar Rodríguez Rojo (coord.)
  3. Alfonso San Miguel Ayanz (coord.)
  4. Federico Fernández González (coord.)
  5. Sonia Roig Gómez (coord.)

Publisher: Sociedad Española para el Estudio de los Pastos

ISBN: 978-84-614-8713-4

Year of publication: 2011

Pages: 401-406

Congress: Sociedad Española para el Estudio de los Pastos. Reunión Científica (50. 2011. null)

Type: Conference paper

Abstract

The objective of this study was to utilize the Cornell net carbohydrate and protein system(CNCPS) nitrogen fractions as a basis for estimating forage rumen undegraded protein (RUP) contents.Twenty- nine different herbage samples of irrigated fertilized meadow located at themountain of León were analyzed for: total nitrogen (total N), nitrogen in trichloroacetic acid precipitatedmatter (NTCA), borate-phosphate buffer insoluble nitrogen (BFIN), neutral-detergent insolublenitrogen (NDIN) and acid-detergent insoluble nitrogen (ADIN). These data were used tocalculate the partition of nitrogen fractions proposed by the Cornell Net Carbohydrate and ProteinSystem (CNCPS): A (total N- NTCA), B1 (NTCA-BFIN), B2 (BFIN-NIDN), B3 (NIDN-ADIN) and C(ADIN). Herbage samples were also incubated in situ and rumen undegraded protein (RUP) were estimated at 0·02/h, 0·04/h, 0·06/h and 0·08/h ruminal passage rates (RUP2, RUP4, RUP6 andRUP8, respectively). Multiple regression equations based on B2, B3 y C CNCPS fractions onlyexplain 56·2, 64·5, 67·2 and 68·8 %, respectively, of the variation in RUP2, RUP4, RUP6 andRUP8.