Avances de la técnica de preselección del sexo en el ganado porcino mediante separación de espermatozoides X e Y por citometría de flujo

Resum

Flow cytometric sorting technology based on differential DNA content between X- and Y- spermatozoa is the only effective procedure for achieving offspring of the desired sex. In swine production the application of this method could improve significantly the productive benefits by planning the insemination to produce a specific sex. A detailed knowledge of aspects related to the efficiency of the sperm sorting technique and to aspects related with effects of sorting procedure on the viability and penetration ability of pig oocytes of flow sorted boar spermatozoa, and of the potential damaging effect of the procedure on sperm DNA is necessary to obtain optimal results. This thesis presents the results of experiences about, the usefulness and efficiency of this technology, followed by the analysis of sorted boar spermatozoa motility, viability, penetration ability. Results of mutagenic effect of Hoechst 33342 staining and sorting on sorted boar spermatozoa are also included.