Transcriptome profiling of lentil in response to Ascochyta lentis infection

  1. García-García, Pedro 1
  2. Vaquero, Francisca 1
  3. Vences, F. Javier 1
  4. Sáenz de Miera, Luis E. 1
  5. Polanco, Carlos 1
  6. González, Ana I. 1
  7. Horres, Ralf 2
  8. Krezdorn, Nicolas 2
  9. Rotter, Björn 2
  10. Winter, Peter 2
  11. Pérez de la Vega, Marcelino 1
  1. 1 Universidad de León, Area de Genética, Dpto. de Biología Molecular, 24071 León
  2. 2 GenXPro GmbH, Altenhöferallee 3, 60438, Frankfurt am Main
Journal:
Spanish journal of agricultural research

ISSN: 1695-971X,  2171-9292

Year of publication: 2019

Volume: 17

Issue: 4

Type: Article

DOI: 10.5424/SJAR/2019174-14982 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Spanish journal of agricultural research

Sustainable development goals

Abstract

Aim of study: The purpose was to identify some general and genotypic-specific defense responses, in order to obtain a set of candidate genes presumably involved in the resistance.Area of study: The experiment was carried out in León, Spain.Material and methods: We have analyzed the response of three lentil genotypes to Ascochyta lentis (isolate AL 84) at transcriptomic level using the Massive Analysis of cDNA Ends (MACE) technique: the susceptible cultivar 'Lupa', the moderately resistant 'ILL5588' and the resistant wild accession 'BG 16880' (L. culinaris subsp. orientalis).Main results: MACE results yielded a total of 50,935 contigs. The average number of detected contigs in each of the six samples was approximately of 40,000. In response to Ascochyta infection, the jasmonic acid pathway and the lignin biosynthesis were up-regulated in resistant genotypes, while they were down-regulated in the susceptible one. The response to chitin, the salicylic pathway and the auxin response were activated only in the resistant L. c. culinaris genotype, while the giberellin synthesis was only induced in the susceptible L. c. culinaris cv. 'Lupa'. A set of 18 lentil gene sequences putatively involved in the response to the pathogen were validated by RT-qPCR.Research highlights: It can be concluded that in response to the infection by Ascochyta, the lignin biosynthesis and the JA pathway were critical for the resistance, while the giberellin synthesis seems to be related with susceptibility to the pathogen.

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Funding information

Spanish Ministerio de Econom?a y Competitividad (co-financed with FEDER funds). AGL2013-44714-R. Consejer?a de Educaci?n, Junta de Castilla y Le?n, Spain. LE113G18.

Funders

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