"Magnifección" ¿una solución a la producción de anticuerpos recombinantes en situaciones de epidemia/pandemia?

  1. Isis Asensio García
  2. María Luz Centeno Martín
Revista:
AmbioCiencias: revista de divulgación

ISSN: 1988-3021

Año de publicación: 2016

Número: 14

Páginas: 19-38

Tipo: Artículo

DIALNET GOOGLE SCHOLAR lock_openBULERIA editor

Otras publicaciones en: AmbioCiencias: revista de divulgación

Resumen

Ciertas situaciones de emergencia, como la provocada por el último brote epidémico del virus del Ébola, crean la necesidad de producir, rápidamente y a gran escala, antígenos y anticuerpos útiles en la elaboración de vacunas. La Biotecnología cuenta con varios sistemas de producción de proteínas recombinantes, pero no todos son capaces de responder eficientemente a ese tipo de demandas. Una buena alternativa es el empleo de las plantas como biofactorías. Existen varios métodos para lograr la expresión heteróloga de proteínas en plantas, desde los utilizados para obtener plantas transgénicas, hasta la infección con vectores virales que da lugar a elevados niveles de expresión transitoria. Uno de ellos es la “magnifección”, que combina el uso de vectores virales deconstruidos, y clonados entre los bordes derecho e izquierdo del T-ADN de una cepa de Agrobacterium , con la agroinfiltración realizada a escala industrial en plantas cultivadas en invernadero. Proporciona rendimientos de producción de hasta 5 g de proteína recombinante por kg de biomasa en solo 4-10 días. La metodología se ha aplicado para obtener los anticuerpos de “ZMapp”, el suero que ha dado buenos resultados en el tratamiento de infecciones por el virus del Ébola en humanos, asícomootras proteínas de interés terapéutico.

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Fuente de los datos: Dialnet