Evolución radiográfica del implante de células mesenquimales de tejido adiposo diferenciadas a osteoblastos en la reparación de defectos óseosestudio experimental en ovejas
- Andrés Saldaña Díaz
- Luis R. Ramos Pascua
- M. López Laguna
- Francisco Javier Iglesias Muñoz
- José Manuel Gonzalo Orden
- José Antonio Rodríguez-Altónaga Martínez
- Rafael González Celador
ISSN: 1888-6116
Year of publication: 2010
Volume: 21
Issue: 3
Pages: 192-198
Type: Article
More publications in: Trauma
Abstract
Objective: To investigate the radiographic progress of the implant of adipose tissue-derived mesenchymal cells differentiated to osteoblasts in cavitary defects of sheep femur. Material and Methods: A study was conducted on 100 agricultural workers performing 19 tasks (planting-6, pick-5, and other cleaning-5-3). The physiological assessment consisted of determining the physical capacity through the application of the test step. During the conduct of work, we evaluated the heart rate, oral temperature, the rate of sweats time and energy expenditure. Biomechanical analysis was performed to evaluate the position, strength and repetition of actions using the method MODSI. Patients and methods: A cavitary defect was performed in the bilateral medial femoral condyle in 22 Asaaf sheep aged 3-5 years. Mesenchymal stem cells obtained from adipose tissue, differentiated into osteoblasts, platelet-rich plasma and lyophilised bone heterograft, were implanted. Radiographic controls were performed at 4 and 12 weeks, evaluating bone growth according to the Lane and Shandu scale. Results: Bone formation or growth were not greater than 25% in any of the groups. A greater bone growth was seen at 12 weeks than at 4 weeks, with no statistically significant differences between the groups. Conclusions: Radiographic control of the progression of cavitary bone defects treated with stem cells differentiated to osteoblasts is not a reliable method at 4 or at 12 weeks of implantation