Genetic diversity among Spanish pea (Pisum sativum L.) landraces, pea cultivars and the World Pisum sp. core collection assessed by retrotransposon-based insertion polymorphisms (RBIPs)

  1. Martín Sanz, Alberto
  2. Caminero Saldaña, Constantino
  3. Jing, Runtian
  4. Flavell, A.
  5. Pérez de la Vega, Marcelino
Revista:
Spanish journal of agricultural research

ISSN: 1695-971X 2171-9292

Año de publicación: 2011

Volumen: 9

Número: 1

Páginas: 166-178

Tipo: Artículo

DOI: 10.5424/SJAR/20110901-214-10 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

Otras publicaciones en: Spanish journal of agricultural research

Resumen

Se ha estudiado un total de 122 accesiones silvestres y cultivadas de Pisum sp. usando marcadores basados en polimorfismos de inserción de retrotransposones (RBIP). Las accesiones de Pisum incluyen materiales silvestres y cultivados (cultivares y variedades locales) de la colección nuclear mundial del John Innes Centre (JI) representando a todos los taxones generalmente reconocidos de Pisum, variedades locales de la colección nuclear española, y por último algunas variedades comerciales de guisante ampliamente cultivadas en España. Para el análisis genético se usaron 18 loci RBIP. El polimorfismo general detectado con los marcadores RBIP fue alto y todas las muestras, excepto dos pares, pudieron ser identificadas por un patrón particular de marcadores. Análisis de componentes principales y filogenéticos discriminaron claramente P. fulvum y P. abyssinicum entre ellas y de P. sativum, mientras que las muestras de P. humile y P. elatius se mezclaban con las de otros taxones en distintos grupos. Esto apoya la existencia de tres especies en el género Pisum (P. abyssinicum, P. fulvum y P. sativum). Los resultados indican que la colección nuclear española de guisante mantiene una variabilidad relativamente elevada que está sólo parcialmente representada en los cultivares generalmente sembrados en España. Por tanto, las variedades locales españolas representan aún una fuente de variabilidad genética para la mejora de nuevos cultivares.

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