Caracterización molecular y funcional de proteínas de "Schistosoma bovis" implicadas en la regulación de los mecanismos hemostáticos del hospedador

Laburpena

[EN] Schistosomiasis is an important health and veterinary problem in many tropical and subtropical areas of the world. Schistosoma bovis is a ruminant blood parasite, considered one of the main causes of schistosomiasis animal. This parasitic disease that affects 150 million animals in Asia and Africa and is also present in our country, causing significant losses in livestock farms to reduce the growth rate of infected animals and increase susceptibility to other diseases themselves. Besides its importance veterinary, S. bovis is a good model for studying human schistosomiasis, given the phylogenetic proximity between the species of the genus. To survive in the vascular bed of the host, S. bovis should neutralize not only the immune response but also hemostatic mechanisms of the host, for which you must have the appropriate anti-hemostatic molecules. Despite its importance, anti-hemostatic molecules of schistosomes have hardly been studied. The few details known about the interaction between S. bovis and the hemostatic system of their hosts are those obtained by our research group in previous studies. These studies found that S. bovis manipulates the host fibrinolytic system by fixing and plasminogen activation and identified ten plasminogen binding proteins, and other proteins that could also be related to the regulation of host hemostatic mechanisms, including annexin and SB22. June. The aim of this thesis was the molecular and functional characterization of five proteins of S. bovis, for their interest as potential regulators of host hemostatic mechanisms. The five selected proteins include enolase, glyceraldehyde-3-phosphate dehydrogenase and fructose-1 ,6-bisphosphate aldolase for its expected function as ligands fibrinolytic plasminogen and annexin and antigen for their potential Sb22.6 anticoagulant functions . The molecular and functional characterization of these molecules has not only enabled a deeper understanding of the biology of the parasite and its relationship with the host, but, since neutralization of such molecules antihemostáticas could result in a disruption of the parasite's survival opens new perspectives for the development of vaccines or drugs against S. bovis.