Photobacterium damselae subsp. damselaeanalysis of the genetic diversity and characterization of a conserved two-component system regulating virulence

  1. Terceti, Mateus de Souza
Supervised by:
  1. Carlos Rodríguez Osorio Director

Defence university: Universidade de Santiago de Compostela

Fecha de defensa: 13 December 2018

Committee:
  1. Jesús Manuel Aparicio Fernández Chair
  2. Trinidad de Miguel Bouzas Secretary
  3. Sandra Manuela Rodrigues Sousa Committee member

Type: Thesis

Teseo: 586473 DIALNET lock_openMINERVA editor

Abstract

Photobacterium damselae subsp. damselae is an emerging pathogen of marine aquaculture. By combining phenotypic and genotypic data, and comparative genomics analyses, we here show that this pathogen exhibits a high genetic diversity and that outbreaks in fish are caused by multiclonal populations. We identified and characterized the RstAB two-component signal transduction system, a major positive regulator of virulence and of many aspects of cell physiology in this bacterium. The RstAB regulon comprises the major cytotoxins Dly, PhlyP and PhlyC, as well as hitherto unreported proteins secreted by the type two secretion system (T2SS), that may constitute novel virulence factors in this pathogen.