Cloning of AFLP markers detected by fluorescence capillary electrophoresis

  1. Polanco, C. 1
  2. González, A.I. 1
  3. De La Puente, R. 1
  4. Somalo, S. 1
  5. Ruiz, María Luisa. 1
  1. 1 Universidad de León

    Universidad de León

    León, España


Plant Molecular Biology Reporter

ISSN: 0735-9640

Year of publication: 2005

Volume: 23

Issue: 3

Pages: 271-277

Type: Article

DOI: 10.1007/BF02772757 SCOPUS: 2-s2.0-33645555967 GOOGLE SCHOLAR

More publications in: Plant Molecular Biology Reporter


The available methods Lo isolate specific amplified fragment length polymorphism (AFLP) markers can be used only if markers are detected by radioactive labeling, silver staining, or ethidium bromide staining; these methods are useless if modem and automated genetic analyzers are used to detect AFLP markers by fluorescent labeling. We have developed a method that allows for isolation and cloning of specific AFLP markers obtained with a laser-induced fluorescence capillary electrophoresis system. This procedure has been tested on 5 Arabidopsis thaliana polymorphic AFLP markers, and the nucleotide sequences obtained from these cloned markers were identified and located in the Arabidopsis genome. © 2005 International Society for Plant Molecular Biology.